Purification, properties and glycoprotein nature of arylsulfatase A from sheep brain.
نویسندگان
چکیده
A simple and rapid method for the purification of arylsulfatase A (EC 3.1.6.1) from sheep brain has been developed. This includes the concanavalin A-Sepharose affinity chromatography and the pH-dependent polymerization and depolymerization of the enzyme. By these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. Sheep brain arylsulfatase A has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. The constituent neutral sugars were identified as glucose and mannose.
منابع مشابه
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ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 403 1 شماره
صفحات -
تاریخ انتشار 1975